Our laboratory is equipped with cutting-edge technology and equipment to support our research on protein misfolding, peptide engineering, proteomics, and structural analysis. We have dedicated spaces for molecular biology, biochemistry, cell culture, peptide synthesis, and biophysical analysis.
Biochemistry Laboratory
Our biochemistry lab is equipped with state-of-the-art instruments for protein purification and characterization, including:
- HPLC System for protein purification
- Ultra-centrifuges
- Spectrophotometers
- Circular Dichroism Spectrometer
- Fluorescence Spectrometer
- Microplate Reader
- Freeze Dryer
- Rotary Evaporator
- Thermo Shaker
- Vacuum Concentrator
- Semi-micro balance
- 2D Gel Electrophoresis
- Dot Blot apparatus
Proteomics and Peptide Synthesis Facility
The Proteomics and Peptide synthesis facility has been established with an aim to cater services for research in the field of Proteomics and Peptide synthesis. This facility mainly focuses on:
Proteomics
- Protein Identification
- Protein profiling (with and without fractionation)
- Post translational modifications
- Protein mapping/maximum sequence coverage
- Identification of N-terminus by mass spectrometry
- Molecular weight measurement of intact proteins
Peptide Synthesis
- Synthesis of Peptide (up to 20 amino acids)
- Custom peptide design for therapeutic applications
- Modification of peptides with functional groups
- Peptide purification and characterization
Key Equipment
- MALDI-TOF Mass Spectrometer
- Microwave Peptide Synthesizer
- Automated Chromatography System
- Preparative/Analytical HPLC
- Ion Trap Mass Spectrometer with UHPLC
- Semi-micro balance
- Dot Blot apparatus
- Thermo Shaker
Peptide Engineering Laboratory
Our peptide engineering laboratory focuses on designing novel peptides with specific functions:
- Computational tools for peptide design
- Structure prediction software
- Peptide-protein interaction analysis
- Molecular docking simulations
- Structure-activity relationship studies
- Peptide stability assessment
Cell Culture Facility
Our cell culture facility includes:
- Laminar flow hoods
- CO₂ incubators
- Inverted phase-contrast microscope
- Inverted Fluorescence Microscope with live-cell imaging capabilities
- Cell counter and viability analyzer
- Centrifuge
Advanced Imaging
We utilize advanced imaging techniques to visualize protein aggregates and peptide structures:
- Confocal Microscope
- Atomic Force Microscope (AFM)
- Transmission Electron Microscope (TEM)
- Super-resolution microscopy
Computational Resources
We have access to high-performance computing facilities for molecular dynamics simulations and data analysis:
- Access to University's High-Performance Computing (HPC) cluster
- Workstations with GPU acceleration for molecular dynamics
- Software licenses for structural biology and simulation tools
- Proteomics data analysis software
- Peptide design algorithms